Table 5.
Generated plasmids and used strains in this work
| Name | Source |
|---|---|
| Plasmids | |
| BB1_pIDTSmart(KanR)_FS1_114p_FS2 | Sarkari et al. [54] |
| BB1_pIDTSmart(KanR)_FS2_amilCP_FS3 | Sarkari et al. [54] |
| BB1_pIDTSmart(KanR)_FS3_BBa_B1001_FS4 | Sarkari et al. [54] |
| BB1_pIDTSmart(KanR)_FS2_acs_FS3 | This work |
| BB1_pIDTSmart(KanR)_FS2_acs_L641P_FS3 | This work |
| BB2_pUC(AmpR)_LinkerA_FS1_FS4_LinkerB | Sarkari et al. [54] |
| BB2_pUC(AmpR)_LinkerA_114p_acs_LinkerB | This work |
| BB2_pUC(AmpR)_LinkerA_114p_acs_L641P_LinkerB | This work |
| BB3_pUC(KanR)_LinkerAD | Sarkari et al. [54] |
| BB3_pUC(KanR)_LinkerA_114p_acs_BBa_B1001_LinkerB | This work |
| BB3_pUC(KanR)_LinkerA_114p_acs_L641P_BBa_B1001_LinkerB | This work |
| Strains | |
| Escherichia coli BL21 (DE3) | New England Biolabs |
| Escherichia coli W DSM 1116 | DSMZ |
BB backbone; 114p BBa_J23114, constitutive promoter from Anderson promoter library; FS fusion site; BBa_B1001 artificial terminator