Fig 3.

Enforced expression of PDE4a induced EMT phenotype of Huh7 cell. A. Transfection of PDE4a expressing plasmid resulted in up-regulation of PDE4a in Huh7 cells; B. As assessed by MTT assay, enhanced expression of PDE4a increased the cell viability of Huh7 cells; C. ELISA assay displayed that there was magnificently more BrdU incorporation found in Huh7 PDE4a cells than Huh7 Vector cells; D. As examined by scratch wound healing assay, cell migration of Huh7 cells was strengthened by over-expression of PDE4a significantly; E. Transwell chamber coated with Matrigel matrix was used to measure the cell invasion ability and it was found that over-expression of PDE4a enhanced invasion capacity of Huh7 cells clearly; F. Enforced expression of PDE4a repressed E-cadherin expression and up-regulated the expression of N-cadherin, TWIST, Vimentin and TWIST in Huh7 cells. Supplementary fig. 1 presented the expression of E-cadherin, N-cadherin, Vimentin and TWIST examined by Western immunoblotting semiquantitatively.