Fig 4.

Knockdown of PDE4a attentuated the malignant behaviors and reversed EMT phenotype in MHCC97h cells. A. As examined by both qRT-PCR and Western immunoblotting assays, PDE4a expression was found significantly decreased by transfection of PDE4a siRNA sequences; B. MTT assay showed that silencing PDE4a leaded to repression of cell viability in MHCC97h cells; C. BrdU ELISA assay displayed that knockdown of PDE4a restrained cell proliferation in MHCC97h cells significantly; D. Invasion capacity of MHCC97h cells was repressed clearly by knockdown of PDE4a; E. Silencing PDE4a was found to inhibit expression of both N-cadherin, Vimentin and TWIST, and increase E-cadherin expression by Western immunoblotting assay. Supplementary fig. 2 displayed the expression of E-cadherin, N-cadherin, Vimentin and TWIST examined by Western immunoblotting semiquantitatively.