Table 2.
Cost and time of qRT-PCR, DNA Microarray, NanoString nCounter, Illumina MiSeq RNA-Seq, and Tissue Microarray assay. The prices could vary from different facilities.
| Assay | qRT-PCR | DNA Microarray | NanoString nCounter | Illumina MiSeq RNA-Seq | Tissue microarray & FISH |
|---|---|---|---|---|---|
| Cost on Sample preparation | $0.56 (SYBR)$0.82 (probe)/Singleplex reaction | ~$50/array | $20 | ~$200-300 | $75-2000/array, depending on cancer type |
| Cost on Kit or plate | $1416 (SYBR) $1834 (probe)/100 preps |
~$350/plate | $280-350/1 cartridge for 12 samples (Master Kit); $3600-4800/12 assays (Custom CodeSet) | $1200-2320/~12 reactions | $515 for FISH |
| Cost on Processing | $0.50/sample | $0.025/data point; ~$100/array | $4.17/data point; $35-41.67/sample | $90/sample | $12.50/sample |
| Cost on Data analysis | $55 | ~$100 | $65-250 | $160-175 | $95/slide using 3 antibodies |
| Cost on Instrument | $25 000- $95 000 Or ~$25/run |
$50 000- $110 000 |
$235 000-$285 000 | $128 000 | $55 000- $98 000 |
| Time on Sample preparation | 1 hour | 20 minutes | 5 minutes | 8 hours | 30 minutes |
|
Time on Sample processing |
2-4 hours | 60 minutes to 17-18 hours or overnight | 5 minutes + 2.5 hours | 24 hours | 24 hours |
| Time on Data Analysis | 30 minutes | 1 hour 40 minutes | 5 minutes + 2.7 hours | 3 hours | 6 minutes |