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. 2018 Jun 6;9(13):2317–2326. doi: 10.7150/jca.25427

Figure 5.

Figure 5

MiR-1296 directly regulates SFPQ expression in CRC cells. (A) The complementary sequences of miR-1296 were discovered in 3'UTR of SFPQ mRNA using TargetScan (http://www.targetscan.org) and miRanda (microRNA.org). The mutagenesis was performed in the complementary sites for the seed region of miR-1296 (wt, wild type; mt, mutant type). (B) HCT116 cells were transfected with miR-1296 mimic and miR-1296 inhibitor, respectively. qRT-PCR results indicated that miR-1296 overexpression reduced while miR-1296 silencing increased the expression of SFPQ. n=three independent experiments, *P<0.05 by Student's t-test. (C) MiR-1296 inversely modulated the luciferase activity of plasmids that carried wt 3'UTR of SFPQ rather than mt 3'UTR of SFPQ. n=three independent experiments, *P<0.05 by Student's t-test. (D) Down-regulation of SFPQ mRNA was detected in CRC tissues compared to tumor-adjacent tissues. P<0.05 by Student's t-test. (E) MiR-1296 was negatively correlated with SFPQ mRNA expression in CRC tissues. n=80, P<0.05 by Spearman's correlation test. (F) Different subgroups (low/high SFPQ expression) were divided according to the median expression of SFPQ in the cohort of CRC patients from TCGA database. CRC patients with low SFPQ level had a significant shorter overall survival compared to those with high SFPQ level. P<0.05 by Log-rank test.