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. 2018 Jun 8;14(9):1099–1108. doi: 10.7150/ijbs.26400

Figure 2.

Figure 2

prM-Env, M-Env and Env inhibited PC12 cell growth and induced G2/M arrest in transduced PC12 cells. PC12 cells were transduced with LV-ZIKV-prM-Env, LV-ZIKV-M-Env, LV-ZIKV-Env or LV-eGFP at an MOI of 100. After 48 h of incubation, cells were collected and subjected to MTT assay, flow cytometric analysis, real-time RT-PCR or Western blot. (A) Cell proliferation determined by MTT assay. *: P<0.05 versus with LV-eGFP. (B) Representative data for lentivirus-induced G2/M cell cycle arrest measured by flow cytometry at 48 h after treatment. The y axis represents the cell count, and the x axis represents the DNA content. The experiments were performed at least three times. (C) Data showing percentages of PC12 cells in G2/M phase. *: P<0.05 versus with LV-eGFP. Data are representative of three independent experiments. (D) Cyclin B1 mRNA relative levels in transduced PC12 cells.*: P<0.05 versus with LV-eGFP. (E) Cyclin B1 and CDK1 expression in transduced PC12 cells. Data are representative of three experiments.