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. 2018 Jun 8;14(9):1081–1089. doi: 10.7150/ijbs.24692

Figure 2.

Figure 2

SMC1A phosphorylation promotes HepG2 and Bel7402 cell proliferation and viability. (A) The flag-tagged wild-type or S957DS966D mutants SMC1A plasmid was transiently transfected into HepG2 and Bel7402 cells knocking down endogenous SMC1A. Effect of SMC1A phosphorylation on HepG2 and Bel7402 cell proliferation was determined by CCK8 assay, ***P<0.001 (B) and PCNA protein expression, **P<0.01 (C). SMC1A (S957DS966D) mutant promoted HepG2 and Bel7402 cell viability in response to the increased doses of Doxorubicin treatment, *P<0.05, **P<0.01, ***P<0.001 (D). Data were expressed as means ± SD. WT, wild-type.