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. 2018 Jun 8;14(9):1067–1080. doi: 10.7150/ijbs.23845

Figure 3.

Figure 3

WDR1 positively regulates cell migration, invasion and induces the EMT process in vitro. A: Wound healing assay in A549, H1299 and H226 cell lines. Knockdown of WDR1 (shWDR1) significantly inhibited cell migration (48h) in A549, H1299 and H226 cells, compared with control cells (shCTL). B: Wound healing assay and quantification analysis showed that overexpression of WDR1 (WDR1) increased cell migration (36h) in A549, H1299 and H226 cell lines, relative to the control cells (GFP). C: Matrigel invasion assay and quantification analysis showed that WDR1 depletion greatly inhibited invasive abilities of H1299 and A549 cells. D-E: Western blotting analysis (D) and quantification (E) revealed decreased expression levels of N-cadherin, Vimentin and ZEB1 in shWDR1 cells, and increased level of E-cadherin. Snail and twist were reduced but not significantly in shWDR1 cells. The target protein band is indicated by an arrow. F: Immunofluorescence staining analysis of E-cadherin and N-cadherin in A549 cells. N-cadherin staining was dramatically reduced in shWDR1 cells, while that of E-cadherin was increased and sometimes punctate (arrow). Data are expressed as means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001. Scale bars = 20 μm.