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. 2018 Jun 8;14(9):1067–1080. doi: 10.7150/ijbs.23845

Figure 5.

Figure 5

WDR1 promotes tumor cell proliferation and migration might through actin cytoskeleton-regulated YAP activity. A-D: The protein levels of total YAP and p-YAP were examined by western blotting analysis. Western blotting and quantification data showed that, Wdr1 depletion resulted in a significantly decreased level of total YAP, increased levels of pYAP (S127) and pYAP (S381) (A,B), whereas Wdr1 overexpression increased total YAP and decreased pYAP (S127), pYAP (S381) (C,D). E: Immunoblotting for the indicated proteins. Cell lysates were fractionated into nuclear and cytoplasmic fractions. TBP and GAPDH were shown as loading controls for each fractionation. shWDR1 cells exhibited dramatically decreased nuclear YAP and comparable level of cytoplasmic YAP. Quantitation of the relative protein levels was also shown. F: qPCR analyses of relative normalized expression of YAP target genes CTGF, ANKRD1 and CYR61. The expression levels of the three target genes were significantly decreased in shWDR1 A549 cells. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001.