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. 2018 Jun 25;2018:9086747. doi: 10.1155/2018/9086747

Figure 7.

Figure 7

Andro treatment blocks HG-triggered ROS generation via regulating NOX/Nrf2 redox imbalance in H9c2 cardiomyoblasts. (a and b) DCFH-DA and DHE probes were used to measure the levels of cellular ROS using confocal microscopy. The relative fluorescence intensity was acquired. (c) Western blot analysis was performed to evaluate the levels of Nrf2 and HO-1. (d–f) NADPH oxidases (NOX2, NOX4, and p47phox) mRNA levels were detected by real-time qPCR analysis. NG, 5.5 mM glucose; HG, 25 mM glucose. ∗ P < 0.05 compared to NG; # P < 0.05 compared to HG.