Figure 1.

Effects of PPARβ/δ agonists on Nrf2 expression. (a, c) mRNA and (b, d) protein expression of Nrf2 in HUVECs exposed to low (5 mM, LG) or high glucose (30 mM, HG) for 24 h with or without GW0742 (GW) or L165041 (L) alone or preincubated with the PPARβ/δ antagonist GSK0660 (GSK). mRNA data presented as a ratio of arbitrary units of mRNA (2^−ΔΔCt). All data are mean ± SEM (n = 8), and experiments were repeated at least three times independently. Protein data presented as densitometric values and protein band normalized to the corresponding α-actin; the bands are representative of n = 3–5. ^∗∗P < 0.01 versus LG. ^#P < 0.05 versus HG. †P < 0.05 versus L and GW column, respectively.