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. 2018 Jun 25;2018:4327901. doi: 10.1155/2018/4327901

Figure 5.

Figure 5

Aucubin attenuates oxidative stress and increases thioredoxin (Trx) in vivo and vitro. (a) and (b) Western blot analysis of P67, gp91, SOD, and Trx in the indicated heart tissue post-MI ((a) representative blots and (b) statistical analysis result) (n = 6). (c) NO production in the indicated heart tissue. ∗p < 0.05 versus vehicle-sham and #p < 0.05 versus vehicle-MI. (d) and (e) Western blot analysis of P67, gp91, SOD, and Trx in the aucubin- (50 μM) pretreated H9c2 cardiomyocytes after exposure to hypoxia for 24 h ((a) representative blots and (e) statistical analysis result) (n = 4 sample). (f) ROS level detected by DCF-DA in the indicated group (n = 6 sample). (g) NO production in the indicated group (n = 6 sample). ∗p < 0.05 versus normoxia + PBS and #p < 0.05 versus hypoxia + PBS. All experiments were repeated independently three times.