Figure 2.

FZKA induces lung cancer cell apoptosis. (a–c) Lung cancer cells (H1650, A549, and PC9) were treated with increased concentrations of FZKA for 24 h. Afterwards, cells were harvested for analysis of apoptosis using the Annexin V-FITC/PI Apoptosis Detection Kit as detailed in Materials and Methods. The A3 quadrant (annexin V−/PI−), A4 quadrant (annexin V+/PI−), and A2 quadrant (annexin V+/PI+) of the histograms indicated the percentage of normal cells, early apoptosis, and late apoptosis, respectively. Values in bar graphs were given as the mean ± SD from three independent experiments performed in triplicate. ∗ indicates significant difference as compared to the untreated control group (^∗P < 0.05).