Figure 2.

Linc00473 Promotes Trophoblast Proliferation In Vitro

(A–C) The viability of si-linc00473-transfected HTR-8/SVneo cells (A) or pCDNA3.1-linc00473-transfected JAR (B) and JEG3 (C) cells were analyzed by the MTT assay. (D–F) The proliferation of si-linc00473-transfected HTR-8/SVneo cells (D) and pcDNA3.1-linc00473-transfected HTR-8/SVneo, JAR (E), and JEG3 (F) cells was evaluated by the colony formation assay, and the number of colonies was counted. (G–I) Proliferating HTR-8/SVneo (G), JAR (H) and JEG3 (I) cells are marked with EDU (red) and cell nuclei are stained with DAPI (blue). The data are presented as the mean ± SD of three independent experiments; *p < 0.05 and **p < 0.01.