Figure 5.

Linc00473 Knockdown Affected the Expression of Genes Involved in Cell Proliferation and Migration

(A and B) Gene expression profiling was performed by RNA-seq in HTR-8/SVneo cells transfected with si-linc00473. Differentially expressed genes are shown (A). GO analysis of genes differentially expressed in si-NC- and si-linc00473-transfected cells. Cell growth was among significant biological processes affected by linc00473 depletion in trophoblasts as evidenced by the number of genes with altered expression (B). (C and D) Relative mRNA expression of genes involved in cell proliferation and migration in linc00473-deficient HTR-8/SVneo cells (C) and linc00473-overexpressing JAR cells (D) was analyzed by qRT-PCR. (E and F) TFPI2 protein expression in linc00473-deficient HTR-8/SVneo cells (E) and linc00473-overexpressing JAR cells (F) was assessed by western blotting. The data are presented as the mean ± SD of three independent experiments; *p < 0.05 and **p < 0.01.