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. 2018 Jun 14;9(14):2480–2491. doi: 10.7150/jca.25213

Figure 1.

Figure 1

Cry1 was downregulated in human osteosarcoma tissues and cells and the establishment of Cry1 knockdown Hos and U2os cells. A. The low expression level of Cry1 in human osteosarcoma tissues. B. Cry1 mRNA was highly expressed in human osteoblast cell line hFOB1.19, compared with Hos and U2os human osteosarcoma cell lines. C. Levels of Cry1 protein in the blank Hos and U2os cells, and Western Blot results of Cry1 silencing in the four groups. D. and E. Measurement of Cry1 silencing efficiency in protein and mRNA levels in the Hos and U2os cells. Values are expressed as the mean ± standard deviation. *P<0.05 vs. the control. **P<0.01 vs. the control. ***P<0.005 vs. the control.