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. 2018 Jun 14;9(14):2480–2491. doi: 10.7150/jca.25213

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Proliferation and migration of Hos and U2os cells after inhibiting the Akt/P53/p21 signaling. A. Analysis of indicated proteins in Cry1 silencing osteosarcoma cells after MK-2206 treatment. Data are expressed as the mean ± standard deviation. B. and C. Results of CCK8 assay and colony formation assay shown that enhanced proliferation of the Cry1-shRNA cells was blocked after Akt inhibition. D. and E. Results of transwell assay and scratching assay demonstrated inhibited migration ability of Cry1 silencing osteosarcoma cells. Data are expressed as the mean ± standard deviation. *P<0.05 vs. the control.