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. 2018 Jun 7;8(13):3544–3558. doi: 10.7150/thno.24607

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The subcellular co-localization of Z_{HPV16 E7} affitoxin384 with HPV16 E7 in SiHa cells. SiHa cells that were respectively incubated with Z_wt affitoxin, Z_{HPV16 E7} 384 and Z_{HPV16 E7} Affitoxin 384 were analyzed by indirect immunofluorescence assay. The anti-HPV16E7 rabbit polyclonal antibody and anti-His tag mouse monoclonal antibody were used as primary antibodies. The goat anti-rabbit antibody conjugated with FITC (green) and goat anti-mouse antibody conjugated with alexa fluor 647 (rose red) were used as secondary antibodies. Cell nucleuses were stained with PI (red). The merged images showed the co-localization of Z_{HPV16 E7} affitoxin384 with HPV16 E7 (gray white). Scale bar = 10 μm. White boxes represent selected zoomed area.