Figure 5.

Fluorescence spectra for simultaneous detection of two different DNA targets, including p53 and BRCA1 genes. The concentrations of FAM-labeled p53 probe and Cy5-labeled BRCA1 probe were 1 nM. These two probes were mixed in the presence of different target combinations: (A) p53 gene without BRCA1 gene, (B) BRCA1 gene without p53 gene, and (C) p53 and BRCA1 gene. Fluorescence spectra were collected with the excitation/emission wavelengths of 485 nm/516 nm for FAM, and 633 nm/662 nm for Cy5, respectively.