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. 2018 May 24;8(13):3437–3460. doi: 10.7150/thno.23853

Figure 4.

Figure 4

Turn-ON properties and kinetics of degradation by CTSB of P-GFLG-Cy5 and PCQ conjugates. (A) Cy5 fluorescence is turned-ON upon incubation with CTSB (0.56 U/mL), while in the addition of an inhibitor (CA-074 Me) and in the absence of CTSB, a negligible increase in fluorescence signal was observed. Data represent mean ± SD (n = 3). (B) Representative CRI MaestroTM image of P-GFLG-Cy5 probe incubated with CTSB, and controls (no enzyme, and an addition of CTSB inhibitor, CA-074 Me). (C) Kinetics of the degradation of Turn-ON probes by CTSB. Various concentrations of P-GFLG-Cy5 (red) and PCQ (blue), from 0-60 µM Cy5-eq. concentration, were incubated with CTSB 0.2 U/mL at 37 °C for 24 h, and the initial velocity values (v) were analyzed by GraFit 7.0 software. (D) Table of kinetic parameters of the Turn-ON probes for CTSB (0.2 U/mL). Data represent mean ± SD (n = 3).