Table 2.
The developmental time of H. vigintioctopunctata larvae and pupa reared on different diet by maintaining the concentration of carbohydrates, and then increasing the concentration of protein (p29:c20, p31:c20, p33:c20, and p35:c20)
| Stage | The ratio of protein: carbohydrate | |||
|---|---|---|---|---|
| 29:20 | 31:20 | 33:20 | 35:20 | |
| L1 (d) | 4.84 ± 0.10a,b | 4.74 ± 0.09b | 5.02 ± 0.10a | 4.96 ± 0.08a,b |
| L2 (d) | 3.58 ± 0.08b | 3.82 ± 0.12a,b | 3.80 ± 0.06a | 3.55 ± 0.07b |
| L3 (d) | 5.21 ± 0.14a | 4.68 ± 0.15b | 5.06 ± 0.09a | 5.00 ± 0.13a,b |
| L4 (d) | 10.56 ± 0.41a | 10.07 ± 0.37a | 9.11 ± 0.11b | 9.10 ± 0.14b |
| Pupa (d) | 7.12 ± 0.16a | 6.28 ± 0.10b | 6.08 ± 0.10b | 5.47 ± 0.12c |
| Total larval stage (d) | 23.75 ± 0.67a | 23.19 ± 0.46a | 22.95 ± 0.22a | 22.61 ± 0.27a |
Values are means ± standard errors. Standard errors were evaluated using the bootstrap procedure with 100,000 resampling and different letters in the same row indicated significantly different (P < 0.05) between the ratio of p:c using the paired bootstrap test. (L1, the first instar; L2, the second instar, L3, the third instar, L4, the fourth instar.)