Figure 4.

Sub-retinal injections in adult mice show superior transduction ability of AAV-TT throughout different layers of the retina. GFP expressing AAV2 and AAV-TT were injected contralaterally in adult mice at a dose of 2 × 10⁹ vg per eye (n = 4 per condition). (A) Representative confocal z-projections of transverse sections of the retina taken 4 weeks after sub-retinal injections show that AAV-TT targets a higher number of RPE and photoreceptor cells and mediates increased transgene expression as compared to AAV2. (B) AAV-TT cannot efficiently penetrate the retina when injected via the intra-vitreal route; intra-vitreal injection of AAV2 leads to transduction of retinal ganglion cells. GC = ganglion cell layer; INL = inner nuclear layer; ONL = outer nuclear layer; PS = photoreceptor segments; RPE = retinal pigment epithelium. Scale bar = 200 µm.