Figure 1.

In Vitro Functionality of Pbbz CAR and dnTGF-βRII-T2A-Pbbz CAR

(A) Lentiviral transduction allows for efficient expression of 19bbz, Pbbz alone, or dnTGF-βRII-T2A-Pbbz in primary human T cells. (B) Expression of dnTGF-βRII prevents TGF-β signal induction through Smad2/3. (C) T cells expressing Pbbz specifically lyse PSMA⁺ PC3 cells in 24-hr luciferase-based lysis assays. (D) ELISA determination of the secretion of the latent form of TGF-β by tumor lines. (E) Expression of dnTGF-βRII-T2A-Pbbz enhances antigen-specific proliferation of CAR T cells upon co-culture with PSMA⁺ PC3 cells (y axis log10 scale). The error bars represent ± SD. **p < 0.01, and ***p < 0.001.