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. 2017 Jun 3;73(8):1018–1026. doi: 10.1093/gerona/glx102

Figure 3.

Figure 3.

T cell and T cell subset numbers within the bone marrow of unmanipulated adult and old animals, isochronic and heterochronic parabionts. (A) Data depict numbers of adult or old CD3 T cells in nonsurgical control, isochronic parabionts, and heterochronic parabionts in bone marrow, isolated as in Materials and Methods, and obtained by a combination of Hemavet® counts and flow cytometry. (B) Numbers of adult or old CD8 T cells in nonsurgical control, isochronic parabionts, and heterochronic parabionts in bone marrow (same animals as in A). (C) Numbers of adult or old CD4 T cells in nonsurgical control, isochronic parabionts, and heterochronic parabionts in bone marrow (same animals as in A). Each group represents five to seven mice, pooled from two to three experiments. Significance was determined by Mann–Whitney U-test for adult and old nonsurgical controls, whereas significance for parabionts was determined by two-way ANOVA with Sidak’s post-test. Statistical significance: *p < .05, **p < .01, ***p < 0.001, ****p < 0.0001, and ns (not significant) is not shown.