Figure 4.

Summary of the assignment. (A) Highly degenerate areas of the ¹H-¹⁵NHSQC spectrum of ataxin-3(Q13) annotated with the assignment. (B) Final achieved assignment reported on thesequence of ataxin-3(Q13). The bar colors are associated to the technique used for assignment. The backbone NH pairs of the Josephin domain (residues 1–182) were assigned by comparison with the spectrum of the isolated domain without (gray bars) or with the aid of ¹⁵N-amino acid selective labeling (red bars) or BEST-TROSY HSQC (red stars). Residues assigned by comparison of the NOESY-HSQC of ataxin-3(Q13) with that of the isolated Josephin domain are indicated by blue bars. The C-terminal tail was assigned via HNCACB and CBCAcoNH (black bars), 5D HNcaCONH and HabCabCONH (green bars), selective ¹⁵N-amino acid labeling (red bars), BEST-TROSY-HNCACB (black stars), and single-point mutations (black circles). To see this figure in color, go online