Table 2. Comparison of the Effect of Mutations of Side-Chains near the Active Site of TIM on Catalytic Activity and the Stability of Complexes to PGA Trianion (I3–).
| Enzyme | log (Ki)E | pKEHI | Δ(pK)EHIa | Δlog (kcat/Km)b |
|---|---|---|---|---|
| Wildtype chicken | –1.7 | 10.5 | ||
| Wildtype Tbb | –1.7 | 10.0 | ||
| Wildtype yeast | –1.7 | 10.4 | ||
| TbbP166A | –2.0 | 9.3 | 0.7 | 1.4 |
| yS211A | –2.6 | 8.2 | 2.2 | 1.6 |
| TbbI170A | –2.1 | 7.7 | 2.3 | 1.9 |
| cL7R | –2.6 | 6.7 | 3.8 | 2.4 |
| yY208F | –1.8 | 6.3 | 4.1 | 3.1 |
a
The difference in the pKEHI for each mutant comparing to the wildtype enzyme.
b
Difference in the log(kcat/Km) for each mutant comparing to the wildtype enzyme.