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In situ trypsin digestion of prostate tissue. A tissue core was harvested from a prostate surgery specimen and serial sections were made. a. One section was sprayed with trypsin, and one was used as a control. Tissues were sprayed with CHCA and spectra were collected in reflectron mode. b. one peptide at m/z 1,408.75 was selected for in situ MS/MS and produced a fragmentation spectrum which was identified as corresponding to a peptide of transgelin with a MASCOT ion score of 86
