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. 2018 Jul 3;11:3793–3803. doi: 10.2147/OTT.S165220

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© 2018 Yao et al. This work is published and licensed by Dove Medical Press Limited

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Limonin decreased HK-2 expression in mitochondria.

Notes: (A) HK-2 expression in mitochondria was reduced. After limonin treatment, the cytosolic and mitochondrial fractions were separated, and HK-2 expression was assessed by Western blotting. (B) The interaction between HK-2 and VDAC-1 was decreased. The cell lysates were immunoprecipitated with VDAC-1 antibody and then probed with HK-2 antibody by Western blotting. (C) HK-2 phosphorylation was decreased after limonin treatment. Cell lysates were immunoprecipitated with HK-2 antibody and then detected with PAS antibody to examine HK-2 phosphorylation.

Abbreviations: HK-2, hexokinase-2; VDAC-1, voltage-dependent anion channel 1; PAS, phospho-Akt substrate.