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. Author manuscript; available in PMC: 2018 Jul 9.
Published in final edited form as: Tumour Biol. 2017 Nov;39(11):1010428317705507. doi: 10.1177/1010428317705507

Figure 1.

Figure 1

Proliferation, viability, and migration of conditioned-medium-treated or co-cultured SCC-25 cells. After 3 days of treatment, SCC-25 cells were counted and cell migration was investigated using a scratch assay. (a) The treatment with FIB and mixed-culture CM (p < 0.001) and co-culture (p < 0.01) leads to significantly higher cell numbers compared to control (albumin-medium-treated cells, which was set to 100%). (b) FIB CM and mixed-culture CM significantly (p < 0.001) increased the lateral migration of SCC-25 cells even in the first 24 h. SCC-25 cells treated with SCC-25 CM or co-cultured with FIBs exhibited a significant higher cell coverage than albumin-medium-treated (control) cells (SCC-25 CM: p < 0.001, co-culture: p < 0.01). (c) These effects could also be observed on the images taken every 24 h, where SCC-25 cells migrated toward empty space when treated with FIB CM or mixed-culture CM (CM: conditioned medium; FIB: human gingival fibroblasts; **p < 0.01; ***p < 0.001). Cells treated with FIB CM or mixed-culture CM or co-cultured SCC-25 cells showed elongated, mesenchymal-like morphology, especially in the scratched area (bars: 100 μm).