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. Author manuscript; available in PMC: 2018 Jul 9.
Published in final edited form as: Nature. 2017 Dec 13;552(7685):362–367. doi: 10.1038/nature24633

Figure 1. Measuring the turnover rate and half-life of human YFV-specific CD8 T cells using in vivo deuterium labelling.

Figure 1

a, Design for study 1. b, FACS plots show percentage of tetramer+ CD8 T cells in a representative vaccinee. Graph shows the longitudinal analysis of YFV-specific tetramer+ CD8 T cells in the blood after vaccination (solid lines, n = 12). The period of D2O intake is shown as a shaded blue area; the kinetics of YFV-17D genomes in the plasma from a separate group of vaccinees is shown as a dashed line. APC, allophycocyanin, PerCP, peridinin chlorophyll. c, Dilution curves of the deuterium enrichment in A2-NS4B214 tetramer+ CD8 T cells sorted at various times post-vaccination, in study 1 (n = 10). The average T1/2 of label enrichment was calculated after wash-out of deuterium from body water (day 42 onwards). Data for each vaccinee are shown as a percentage of the label incorporation seen at the end of the heavy water intake period.