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. Author manuscript; available in PMC: 2018 Jul 9.
Published in final edited form as: Nature. 2017 Dec 13;552(7685):362–367. doi: 10.1038/nature24633

Extended Data Figure 9. Open chromatin sites near the TSSs of BCL-2 and IL-7R (CD127) in YFV-specific effector and memory CD8 T cells.

Extended Data Figure 9

Plots show traces of chromatin openness (y axis) at the IL-7R and BCL-2 loci (x axis). The dashed rectangles represent the ATAC–seq peaks identified as open that were significantly different between at least two subpopulations. The RefSeq gene tracks are represented in the bottom row. We identified open sites at the TSS as well as immediately upstream and downstream of the TSS for IL7R in naive cells that all transiently lost accessibility in effector CD8 T cells and then opened in the YFV-specific memory CD8 T cells. A similar temporary pattern was seen for open chromatin regions in the BCL2 gene. We found an accessible site downstream of the BCL2 TSS that partially closes in effector cells but then regains openness to an even greater extent in memory cells than in naive cells, consistent with previous observations that memory cells are characterized by increased BCL-2 expression.