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. 2018 Jul 9;13(7):e0200369. doi: 10.1371/journal.pone.0200369

Table 1. Description, accession number of the reference gene, size and GC percentage of PCR products, qRT-PCR efficiency, and correlation coefficient (R2) of the selected reference genes.

Symbol Accession no. size (bp)* GC (%) Efficiency (%) R2
rp49 AF441189 150 (442) 37.6 87.6 0.9980
rpL32 XM006564315 181 33.1 105.3 0.9897
rpS18 XM625101 152 (446) 35.5 107.6 0.9999
tbp XM623085 99 (177) 34.3 106.8 0.9962
tub XM396338 145 40 82.6 0.9993
gapdh XM393605 188 43.1 95.5 0.9970
ace2 NM001040230 139 65.5 100.6 0.9969

* Number in brackets indicate the size of PCR products amplified with genomic DNA