Table 1. Description, accession number of the reference gene, size and GC percentage of PCR products, qRT-PCR efficiency, and correlation coefficient (R2) of the selected reference genes.
Symbol | Accession no. | size (bp)* | GC (%) | Efficiency (%) | R2 |
---|---|---|---|---|---|
rp49 | AF441189 | 150 (442) | 37.6 | 87.6 | 0.9980 |
rpL32 | XM006564315 | 181 | 33.1 | 105.3 | 0.9897 |
rpS18 | XM625101 | 152 (446) | 35.5 | 107.6 | 0.9999 |
tbp | XM623085 | 99 (177) | 34.3 | 106.8 | 0.9962 |
tub | XM396338 | 145 | 40 | 82.6 | 0.9993 |
gapdh | XM393605 | 188 | 43.1 | 95.5 | 0.9970 |
ace2 | NM001040230 | 139 | 65.5 | 100.6 | 0.9969 |
* Number in brackets indicate the size of PCR products amplified with genomic DNA