Table 2. Average expression stability values of the five reference genes in forager, nurse, and integration of two tasks calculated by geNorm and NormFinder.
Program | geNorm | NormFinder | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample | Forager | Nurse | Forager + Nurse a | Forager | Nurse | Forager + Nurse a | |||||||||
Ranking | gene | Average M value | gene | Average M value | gene | Average M value | Gene | Stability value | Standard error | Gene | Stability value | Standard error | Gene | Stability value | Standard error |
1 | rpS18 | 0.843 | rpS18 | 0.866 | rpS18 | 0.84 | gapdh | 0.021 | 0.027 | gapdh | 0.025 | 0.03 | gapdh | 0.022 | 0.019 |
2 | gapdh | 1.017 | gapdh | 1.072 | gapdh | 1.021 | rpS18 | 0.062 | 0.02 | rpS18 | 0.072 | 0.025 | rpS18 | 0.067 | 0.014 |
3 | rpL32 | 1.103 | rpL32 | 1.142 | rpL32 | 1.104 | tbp | 0.077 | 0.021 | tbp | 0.085 | 0.023 | tbp | 0.082 | 0.015 |
4 | tbp | 2 | tbp | 2.107 | tbp | 2.021 | rpL32 | 0.096 | 0.024 | rpL32 | 0.1 | 0.022 | rpL32 | 0.096 | 0.017 |
a Raw data from forager and nurse were combined and expression stabilities of each gene was calculated using geNorm and NormFinder.