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. 2018 Jul 9;13(7):e0200343. doi: 10.1371/journal.pone.0200343

Fig 3. A,B. Phospho-AKT-Ser473 Western blot analysis of lymphatic endothelial cells.

Fig 3

Note that phospho-AKT-Ser473 (pAKT) is highly expressed in lymphatic malformation-derived LECs (Ly-LEC1, 2, 10, and 12), but not in normal HD-LECc3 and c4. Total AKT (tAKT) is found in all cell lines. Antibodies against α-tubulin were used as loading control.