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. 2018 Jul 9;13(7):e0200343. doi: 10.1371/journal.pone.0200343

Fig 4. Phospho-ERK Western blot analysis of lymphatic endothelial cells.

Fig 4

Note that phospho-ERK (pERK) is highly expressed in lymphatic malformation-derived Ly-LECs (Ly-LEC1, 10, and 12), but not in normal HD-LECc3 and c4. Ly-LEC2, which contain a Glu109del in PIK3CA, are similar to normal LECs, which contain clear levels of total ERK (tERK). Antibodies against α-tubulin were used as loading control.