Relative lipid composition (moles per 100 moles) of pooled gallbladder bile (n=5 per group) at each time point (0, 14, 28, and 56 days on the lithogenic diet) is plotted on condensed phase diagrams in which regions A to E with different crystallization sequences have been described elsewhere (27). With passage of time, relative biliary lipid composition of pooled gallbladder bile gradually moves upward and to the right and enters the crystallization region C from the micellar zone in three groups of mice. This indicates that gallbladder bile reaches supersaturation not only in WT mice, but also in ABCG5(−/−)/G8(−/−) and ABCG8 (−/−) mice after 14 days on the lithogenic diet.