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a). Agarose beads were separately labeled with recombinant mCerulean3 or mVenus. Imaging was performed using collection conditions specific for mCerulean3 or mVenus. b). The fluorescence intensity above background was measure for CFP and YFP labeled beads in each channel (n=10 beads per fluorescent protein; ****, p < 0.0001 by t-test.
