Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers |
|---|---|---|---|
| Cell Line (Homo sapiens) |
HEK293-T | RRID:CVCL_0063 | |
| Cell Line (Homo sapiens) |
HeLa | RRID:CVCL_0030 | |
| Cell Line (Mus musculus) |
Min6 | RRID:CVCL_0431 | |
| Cell Line (Rattus norvegicus) |
REF-52 | RRID:CVCL_6848 | |
| Chemical Compound, Drug |
Forskolin | LC Laboratories | F9929 |
| Chemical Compound, Drug |
3-Isobutyl-1-methylxanthine (IBMX) |
Sigma | I5879 |
| Chemical Compound, Drug |
H-89 | LC Laboratories | H5239 |
| Chemical Compound, Drug |
Epidermal Growth Factor (EGF) |
Sigma | E9644 |
| Chemical Compound, Drug |
U0126 | Sigma | U120 |
| Chemical Compound, Drug |
Phorbol 12-Myristate 13-Acetate (PMA) |
LC Laboratories | P-1680 |
| Chemical Compound, Drug |
Tetraethylammonium chloride (TEA) |
Sigma | T2265 |
| Chemical Compound, Drug |
ionomycin | EMD Millipore | 407951 |
| Chemical Compound, Drug |
thapsigargin | Cayman Chemical | 10522 |
| Chemical Compound, Drug |
histamine | Sigma | H7250 |
| Commerical Kit | Lipofectamine-2000 | Invitrogen | 11668019 |
| Commerical Kit | Polyjet | SignaGen | SL100688 |
| Recombinant DNA Reagent |
pCDNA3 AKAR4 | PMID: 20838685 | |
| Recombinant DNA Reagent |
pCDNA3 EKAR-EV | PMID: 21976697 | |
| Recombinant DNA Reagent |
pCDNA3 CKAR1 | PMID: 12782683 | |
| Recombinant DNA Reagent |
FRET MLCK sensor | PMID: 15071183 | |
| Recombinant DNA Reagent |
pCDNA3 YC3.6 Cameleon | PMID: 10051607 | |
| Recombinant DNA Reagent |
pCDNA3 ICUE3 | PMID: 19603118 | |
| Strain (Escherichia coli) |
BL-21 Codon Optimized Plus |
New England Biolabs | C2527 |
| Software | FIJI | RRID:SCR_014294 | |
| Software | MetaFluor | RRID:SCR_002285 | |
| Software | Micromanager | RRID:SCR_000415 | |
| Software | Zeiss Actiovision | RRID:SCR_002677 | |
| Software | MATLAB | RRID:SCR_001622 | |
| Software | GraphPad Prism | RRID:SCR_002798 |