Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (mouse cell line) |
JM8.N4 mouse ES cells |
KOMP repository | RRID: CVCL_J962 | Parental cell line used for all genetic manipulations |
| Antibody | TBP | Abcam | Abcam #ab51841; RRID:AB_945758 |
1:250 for Western; 10 μg for ChIP |
| Antibody | N-20 RNA Polymerase II |
Santa Cruz | Santa Cruz #sc-899; RRID:AB_632359 |
1:250 for Western |
| Antibody | 8WG16 Pol II | Santa Cruz | RRID:AB_785522 | 1:250 for Western |
| Antibody | Flag | Sigma Aldrich | Sigma-Aldrich Cat# F3165, RRID:AB_259529 |
1:5000 for Western |
| Cell line (mouse) | Halo-TBP KI C41 | This paper | Halo-TBP KI C41 | Endogneous knock-in of HaloTag to N-ternimal of TBP in JM8.N4 cells |
| Cell line (mouse) | mAID-TBP KI C94 | This paper | mAID-TBP KI C94 | Endogenous knock-in of the minimal auxin inducible degron to N-terminal of TBP in JM8.N4 cells |
| Cell line (mouse) | Halo-Pol II C64 | This paper | Halo-Pol II C64 | Endogenous knock-in of the HaloTag to C-terminal of Rbp1, largest subunit of Pol II, in mAID-TBP C94 cells |
| Commercial assay or kit |
Nextera DNA Library Preparation Kit |
Illumina | FC-121–1030 | ATAC-seq reagent for Tn5 transposition |
| Software, algorithm | Spot-On | doi: 10.7554/eLife. 25776 |
Spot-on software used to analyze spaSPT data |