Intensity frames were extracted from the FLIM images of intestinal crypts shown in
Figure 4 imaged (
A) in vivo through an optical window and (
B) ex vivo for benchmarking against alternative motion correction packages. (i) Frame averaged motion correction results with (
gray) no correction, (
red) ImageJ plugin StackReg, (
purple) python package SIMA and (
blue) Galene. No frames were excluded from the averaging. (ii) Correlation between each frame in the sequence and the reference frame for each package. (iii) Average correlation for each correction package. Results show mean ± SEM. Mouse and organ illustrations were adapted from Servier Medial Art, licensed under the Creative Commons Attribution 3.0 Unported license.