Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Rac1-FRET, C57BL/6 (mixed)) |
Rac1-FRET biosensor mouse |
Johnsson et al. (2014) | Rac1-FRET biosensor mouse |
|
| Strain, strain background (BALB/c-Fox1nuAusb, Mus) |
BALB/c-Fox1nuAusb | Australian BioResources | BALB/c-Fox1nuAusb | |
| Cell line (Mus) | KPC | Morton et al. (2010a) | KPC primary cancer cells |
|
| Antibody | active Rac1-GTP | NewEast Biosciences | RRID:AB_1961793 | 1:400 concentration |
| Recombinant DNA reagent |
Rac1-FRET biosensor | Itoh et al. (2002) | Raichu-1011X ECFP- SEYFP |
|
| Recombinant DNA reagent |
Src-FRET biosensor |
Wang et al. (2005), Vennin et al. (2017) |
Src-FRET biosensor | |
| Chemical compound, drug |
(-)-Scopolamine-N- butylbromide |
Sigma-Aldrich | PubChem:CID_6852391; Sigma:S7882 |
|
| Chemical compound, drug |
Phorbol myristate acetate |
Sigma-Aldrich | PubChem:CID_27924; Sigma:P8139 |
|
| Chemical compound, drug |
Fasudil | Jomar Life Research | PubChem:CID_163751; Jomar:HA-1077 |
|
| Software, algorithm | Matlab R2018a | Mathworks | RRID:SCR_001622 | |
| Software, algorithm | FLIMfit | Warren et al. (2013) | RRID:SCR_016298 |