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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Mol Cell Biochem. 2018 Jan 9;446(1-2):53–62. doi: 10.1007/s11010-018-3272-5

Fig. 4.

Fig. 4

Calcium responses to ATP are attenuated in the presence of the P2X7 antagonist o-ATP. a Pseudocolored images depict intracellular calcium at basal levels and approximately 30 seconds following application of 200 μM ATP. In experimental treatments, cells were pre-incubated in 1 mM o-ATP for 30 minutes prior to imaging. b Percentage of GL261 cells responding to ATP in control solution (n = 5) and when pre-incubated with 1 mM o-ATP (n = 6). Bars indicate standard error of the mean. Star (*) indicates a statistically significant difference between treatments (p < 0.02, Student’s t-test)