Extracellular ATP decreases GL261 cell viability in a dose-dependent manner.
a GL261 cells were incubated for 48 hours in vehicle control or
the indicated concentrations of ATP and cell viability was assessed using the
MTT assay (n = 5 experiments per treatment). Differences among groups
were statistically significant, indicated by star (*) (p < 0.05,
one-way ANOVA). Pairwise comparisons between treatment groups were tested using
the Holm-Sidak method. There was a significant decrease in cell viability when
cells were exposed to 5 mM ATP when compared to control and lower concentrations
of ATP. b GL261 cells were co-treated with ATP and the
P2X7-antagonist o-ATP for 48 hours and cell viability was assessed with MTT
assays. GL261 cells were incubated with 100 μM o-ATP along with either
vehicle control or 200 μM, 1 mM, or 5 mM ATP. The 100 μM o-ATP
+ 5 mM ATP dose showed a decrease in viability as compared with all
other treatment groups (one-way ANOVA, p < 0.05)