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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Mol Cell Biochem. 2018 Jan 9;446(1-2):53–62. doi: 10.1007/s11010-018-3272-5

Fig. 5.

Fig. 5

Extracellular ATP decreases GL261 cell viability in a dose-dependent manner. a GL261 cells were incubated for 48 hours in vehicle control or the indicated concentrations of ATP and cell viability was assessed using the MTT assay (n = 5 experiments per treatment). Differences among groups were statistically significant, indicated by star (*) (p < 0.05, one-way ANOVA). Pairwise comparisons between treatment groups were tested using the Holm-Sidak method. There was a significant decrease in cell viability when cells were exposed to 5 mM ATP when compared to control and lower concentrations of ATP. b GL261 cells were co-treated with ATP and the P2X7-antagonist o-ATP for 48 hours and cell viability was assessed with MTT assays. GL261 cells were incubated with 100 μM o-ATP along with either vehicle control or 200 μM, 1 mM, or 5 mM ATP. The 100 μM o-ATP + 5 mM ATP dose showed a decrease in viability as compared with all other treatment groups (one-way ANOVA, p < 0.05)