Fig. 1.

Evolutionary tree of the α-amylase family GH57. The analyzed set contains 1602 sequences (for details, see Table 1). The tree is based on the alignment of conserved sequence regions (cf. Fig. 2) and calculated using the neighbor-joining method. The picture shows the same tree emphasizing a its division according to individual enzyme specificities and protein groups and b the division reflecting the taxonomic origin—bacteria (blue) and archaea (red). The 27 experimentally characterized GH57 enzymes (cf. Table 1) are signified by black dots in both versions of the tree. GBE α-glucan branching enzyme, AGAL α-galactosidase, APU-CMD amylopullulanase–cyclomaltodextrinase, 4AGT 4-α-glucanotransferase, AAMY α-amylase