Fig. 1. LC–MS and ESI–QTOF–MS analysis of the purified sample from the ethanoic extract of Graviola.

a Base peak intensity chromatogram of the purified sample and b MS spectrum of the peak at 11.68 min. The main peak at m/z 597.63 Da agrees with the expected singly protonated mass of annonacin. The peak at m/z 619.59 Da can be attributed to the sodiated molecular ion of annonacin, while peaks appearing at m/z 579.64 and 561.59 correspond to the loss of one and two molecules of water, respectively. c ESI–QTOF–MS/MS spectrum of the [M+Na]⁺ adduct of annonacin showing the loss of 112 amu corresponding to the loss of the lactonic ring, which has been previously reported^{28, 29}