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. 2018 Jul 9;8:10327. doi: 10.1038/s41598-018-28593-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of FR on plasma ACE, ACE2 and renin activity. Shown is the time course of product formation (A,B) and enzyme activity (C,D,E) for ACE (n = 8/group) (A,D), ACE2 (CT, n = 8; FR = 9) (B,E) and renin (CT, n = 8; FR, n = 10) (C) in the plasma and the ratio of Ang-[1–10] over AGT production (CT, n = 10; FR, n = 11) (F) in CT (grey circle; white bar) and FR (closed circle; black bar) female rats. *P < 0.001 vs. CT by two-way ANOVA and Bonferroni post-hoc test (time; diet); ^‡P < 0.0001 vs. CT, same treatment, by unpaired Student’s t-test. Values are expressed as the mean ± SEM.