Table 2.
Effect of food restriction on plasma angiotensin peptides before and after Ang-[1–10] infusion.
| Peptide | Vehicle infusion | Ang-[1–10] infusion | Vehicle vs Ang-[1–10] | |||||
|---|---|---|---|---|---|---|---|---|
| CT (pg/mL±SEM) | FR (pg/mL±SEM) | P value | CT (pg/mL±SEM) | FR (pg/mL±SEM) | P value | CT P value | FR P value | |
| AGT (ng/mL) | 2086 ± 72 | 2492 ± 24 | <0.001 ‡ | 1810 ± 65 | 2656 ± 70 | <0.001 ‡ | 0.01 # | 0.04 # |
| Ang-[1–10] | 20.1 ± 1.3 | 20.8 ± 2.7 | 0.99 | 70.2 ± 9.5 | 102 ± 8.7 | <0.005 ‡ | <0.001 # | <0.001 # |
| Ang-[2–10] | 5.9 ± 0.6 | 5.0 ± 0.5 | 0.96 | 8.4 ± 0.8 | 16 ± 2.1 | <0.001 ‡ | <0.02 | <0.001 # |
| Ang-[1–9] | <1.7 ± 0.04 | <1.6 ± 0.05 | nd | <1.5 ± 0.1 | <1.4 ± 0.03 | nd | nd | nd |
| Ang-[1–8] | 50.7 ± 4.9 | 64.6 ± 3.5 | <0.05 ‡ | 67.9 ± 7.1 | 107 ± 16 | <0.05 ‡ | 0.055 | <0.01 # |
| Ang-[1–7] | <2.2 ± 0.04 | <2.1 ± 0.05 | nd | <1.6 ± 0.03 | <1.6 ± 0.04 | nd | nd | nd |
| Ang-[2–8] | 11.1 ± 1.2 | 11.5 ± 1.3 | 0.99 | 8.9 ± 1 | 16.0 ± 2 | <0.02 ‡ | 0.26 | 0.12 |
| Ang-[3–8] | 3.7 ± 0.5 | 4.5 ± 0.3 | 0.42 | 3.8 ± 0.3 | 6.1 ± 0.6 | <0.005 ‡ | 0.90 | <0.05 # |
| Ang-[2–7] | <2.3 ± 0.2 | <1.9 ± 0.2 | nd | <1.2 ± 0.04 | <1.1 ± 0.04 | nd | nd | nd |
| Ang-[3–7] | <1.2 ± 0.02 | <1.2 ± 0.02 | nd | <1.2 ± 0.04 | <1.1 ± 0.03 | nd | nd | nd |
| Ang-[1–5] | 2.3 ± 0.2 | 2.0 ± 0.2 | 0.88 | 3.1 ± 0.1 | 4.8 ± 0.6 | <0.01 ‡ | <0.02# | <0.001 # |
Blood angiotensinogen (n = 8/group) and angiotensin (Ang) peptide concentrations before (CT, n = 10; FR, n = 11/group) and after Ang-[1–10] infusion (n = 8/group) in female rats subjected to control (CT) or food restricted (FR) diet for 14 days. ‡P < 0.05 vs CT by two-way ANOVA and Bonferroni post-hoc test (drug; diet); #P < 0.05 vs vehicle, same group, by unpaired Student’s t-test. (<) in front of a number indicates below threshold of signal-to-noise; nd, not detectable.