Fig. 4.

Cell-intrinsic deficiency in iNKT2 and iNKT17 subsets development in mice with Zap70 hypomorphic mutations. a Representative flow cytometry plots showing the frequency of total iNKT cells and iNKT cell subsets in the thymi of 8-week-old C57BL/6, C57BL/6 YYAA, BALB/c, and BALB/c SKG mice. Each genotype was analyzed independently and compared to wild-type control mice of the appropriate background. b, c Summary of the data shown in a with percentages and cell numbers of total iNKT cells and iNKT cell subsets in the various genotypes analyzed. Data are mean ± SD. Significance was assessed using the unpaired t-test. d Analysis of thymic iNKT cell subsets in competitive bone marrow chimera. Congenically marked bone marrow cells from BALB/c wild-type mice (CD45.1) were mixed in a 1:1 ratio with (CD45.2) BALB/c or SKG bone marrow cells and injected into lethally irradiated F1 (CD45.2 × CD45.1) BALB/c mice. The thymi of the chimera were analyzed for iNKT cell subset composition 8–10 weeks post reconstitution and representative flow cytometry plots are shown. After gating on total iNKT cells, the proportion of iNKT cell subsets derived from either CD45.2⁺ or CD45.1⁺ bone marrow cells is shown. e Summary of the data shown in d with ≥5 mice per group. Data are represented as mean ± SD. Significance was assessed using one-way analysis of variance (ANOVA) *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant