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. 2018 Jul 9;9:2650. doi: 10.1038/s41467-018-05026-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Induction of Egr2 and PLZF in pre-selection DP thymocytes stimulated with plate-bound anti-CD3 and anti-Ly108 mAbs is affected by the SKG mutation. a Expression of Egr2 and PLZF proteins in pre-selection BALB/c or SKG DP thymocytes stimulated with media alone (no stim), plate-bound anti-CD3, anti-Ly108, or anti-CD3 + anti-Ly108 for 48 h. At the end of the stimulation, cells were stained using anti-CD4, -CD8, -Egr2, -PLZF mAbs, and a viability dye. The percentages of live cells that are Egr2⁺ and PLZF⁺ are indicated for each stimulatory condition. b Time course of CD4, CD8, Egr2, and PLZF expression in pre-selection BALB/c or SKG DP thymocytes stimulated with plate-bound anti-CD3 + anti-Ly108 mAbs. Data are shown for live cells in each conditions. c Summary of the data shown in b for each stimulatory condition. The percentage of Egr2⁺ cells, the gMFI of Egr2 expression, and the percentage of PLZF⁺ cells are shown. Each experimental condition was tested in duplicate wells and the data are representative of greater than three independent experiments with similar results