Skip to main content
. 2018 Jul 3;9:1414. doi: 10.3389/fmicb.2018.01414

FIGURE 7.

FIGURE 7

Penicillin G induces the chlamydial stress response in all C. suis strains. (A) Shown are bar graphs comparing the average chlamydial inclusion size of Penicillin G (PenG)-exposed cultures to the mock-exposed control (left; mean ± SD) as well as representative immunofluorescence microscopy images of inclusion morphology of both the unexposed control (right, top) and cultures exposed to 1 U/ml of PenG (right, bottom) for strains SWA-14, SWA-86, SWA-141, and S45/6 at 48 h post infection. Two independent experiments were performed (n = 2). Asterisks indicate a statistically significant difference between the PenG-exposed cultures and the mock by both Student’s t-test and the Welch t-test (ns, not significant; ∗p < 0.05; ∗∗p < 0.01). (B) The bar graphs compare the average inclusion forming units per ml (IFU/ml) of unexposed controls (mock) with PenG-exposed (1, 10, and 100 U/ml) cultures (mean ± SD). Cultures continuously exposed to PenG for 96 h (exp) were compared with cultures exposed to PenG for 48 h and then further cultured in PenG-free medium for 48 h (recovery, rec). Shown are the results for strains SWA-14 (top left), SWA-86 (top right), 141 (bottom left), and the reference strain S45/6 (bottom right). Two independent experiments were performed (n = 2).